2 edition of Identification of competence genes in streptococcus mutans by functional genomic analysis found in the catalog.
Identification of competence genes in streptococcus mutans by functional genomic analysis
Written in English
|Statement||by Janet Lee.|
|The Physical Object|
|Pagination||ix, 89 leaves :|
|Number of Pages||89|
We identified a gene (atlA) encoding autolytic activity from Streptococcus mutans Xc. The AtlA protein predicted to be encoded by atlA is composed of amino acids with a molecular weight of , and has a conserved β-1,4- N -acetylmuramidase (lysozyme) domain in the C-terminal portion. Sodium dodecyl sulfate extracts of strain Xc showed two major bacteriolytic bands with molecular. Abstract. AtlA is a major cell-lytic enzyme called autolysin in Streptococcus this study, we identified the atlg gene-encoding autolysin (Atlg), consisting of residues from Streptococcus sobrinus DP, and confirmed lytic activity of recombinant Atlg by zymography of S. sobrinus cells. An atlA-inactivated mutant was constructed in S. mutans Xc, and the atlg gene product was.
Streptococcus mutans is a major pathogen causing human dental caries. As a Gram-positive bacterium with a small genome (about 2 Mb) it is considered a . These methods include ortholog identificat genomic intrinsic feature analy gene evolution r phylogenetic conservat network analysis 41 .
Trigger factor is a ribosome-associated peptidyl-prolyl cis/trans isomerase that is highly conserved in most bacteria. A gene, designated ropA, encoding an apparent trigger factor homologue, was identified in Streptococcus mutans, the primary etiological agent of human dental caries. Inactivation of ropA had no major impact on growth rate in planktonic cultures under the conditions tested. Regulation of competence and gene expression in Streptococcus mutans by the RcrR transcriptional regulator. Mol Oral Microbiol. ; – [PMC free article] Seaton K, Ahn S-J, Sagstetter AM, et al. A transcriptional regulator and ABC transporters link stress tolerance, (p)ppGpp, and genetic competence in Streptococcus mutans. J Bacteriol.
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Streptococcus mutans is the leading cause of dental caries (tooth decay) worldwide and is considered to be the most cariogenic of all of the oral streptococci. The genome of S. mutans UA, a serotype c strain, has been completely sequenced and is composed of 2, base pairs.
It contains 1, ORFs, 63% of which have been assigned putative by: Streptococcus mutans, the primary etiological agent of human dental caries, is an obligate biofilm-forming goals of this study were to identify the gene(s) required for biofilm formation by this organism and to elucidate the role(s) that some of the known global regulators of gene expression play in controlling biofilm by: Streptococcus mutans, the primary etiological agent of human dental caries, is an obligate biofilm-forming bacterium.
The goals of this study were to identify the gene(s) required for biofilm formation by this organism and to elucidate the role(s) that some of the known global regulators of gene expression play in controlling biofilm formation.
In S. mutans UA, the brpA gene (for biofilm Cited by: Transposon mutagenesis coupled with next-generation DNA sequencing (Tn-seq) is a powerful tool for discovering regions of the genome that are required for the survival of bacteria in different environments.
We adapted this technique to the dental caries pathogen Streptococcus mutans UA and identified 11% of the genome as essential, with many genes encoding products required Cited by: Identification of a novel bacteriocin Lin Zeng, Sang-Joon Ahn, Stephen J.
Hagen, Robert A. Burne, Effects of Carbohydrate Source on Genetic Competence in Streptococcus mutans, Applied and Saswati Biswas, Min Zhu, Effects of DNA Methylation on Expression of Virulence Genes in Streptococcus mutans, Applied and Cited by: The frequencies of 21 competence genes were analyzed in 94 genotypes of Streptococcus include those of a main regulatory system (comCDE), structural, and other regulatory orthologues identified in the genome of strain UAPCR and Southern blot analysis revealed that all genes are widespread within the species.
Random mutagenesis of S. mutans. The random mutagenesis of S. mutans was carried out as described previously ().Briefly, we constructed an S. mutans Xc genomic library by inserting a complete Sau3AI digest of the S.
mutans Xc chromosome into the BamHI site of pResEmBBN. pResEmBBN can be used as an integration vector for gene inactivation by a single crossover with the streptococcal. Streptococcus oralis is an early colonizer bacterium in dental plaques and is considered a potential pathogen of infective endocarditis (IE) this study, we built a complete genome map of Streptococcus oralis strain SOT, Streptococcus oralis strain SOD and Streptococcus infantis strain SO and performed comparative genomic analysis among these three strains.
Identification of the streptococcal competence-pheromone receptor. Mol Microbiol. Aug; 21 (4)– Hui FM, Morrison DA. Genetic transformation in Streptococcus pneumoniae: nucleotide sequence analysis shows comA, a gene required for competence induction, to be a member of the bacterial ATP-dependent transport protein family.
The aerotolerant anaerobe Streptococcus pneumoniae is a human pathogen showing high transformability by soluble DNA. Central metabolism in these bacteria is classically described as homolactic and genome analysis revealed the absence of most of the genes involved in aerobic respiration (29 genes searched for), the tricarboxylic acid (TCA) cycle, and gluconeogenesis ().
Lee, J.H., et al. Genetic transformation in Streptococcus mutans: identification of competence genes by functional genomic analysis. Abstracts of the st Annual General Meeting of the American Society for Microbiology. Orlando, Florida, USA.
(Abstr. As with many other gram-positive organisms, the genome of Streptococcus pneumoniae proved difficult to sequence. The majority of insertion sequence (IS) elements have undergone insertions, deletions, and/or point mutations that result in frame shifted or otherwise nonfunctional transposase genes.
A primary role for the numerous repeats might be their potential contribution to genomic. The frequencies of 21 competence genes were analyzed in 94 genotypes of Streptococcus mutans. These include those of a main regulatory system (comCDE), structural, and other regulatory orthologues identified in the genome of strain UA PCR and Southern blot analysis revealed that all genes are widespread within the species.
Development of Competence for Genetic Transformation of Streptococcus mutans in a Chemically Deﬁned Medium Kunal Desai,a Lauren Mashburn-Warren,b Michael J. Federle,b,c and Donald A.
Morrisona comR genes, and a functional oligopeptide permease (4,17 24). Mashburn-Warren et al. (17) recently reported that a second. Streptococcus gallolyticus subsp. gallolyticus, a member of the group D streptococci, is normally found in the bovine rumen and human gut.
It is an opportunistic pathogen that was recently determined to be a bacterial driver of colorectal cancer, in addition to causing other diseases, such as infective endocarditis, bacteremia, neonatal meningitis, and septicemia.
Abstract. C LARKE () first isolated and described a streptococcus from a human carious lesion and inferred that it was a potential causative agent of dental caries. He named the organism Streptococcus observation went unnoticed for some 35 years until O RLAND () demonstrated that enterococci could cause dental caries in germ-free rats and F ITZGERALD and colleagues (F.
Genomic overview. We began with a bioinformatic analysis of 20 S. sanguinis strains: the oral isolate SK36, which we had sequenced previously (), and 19 other strains that were draft sequenced as part of the Human Microbiome of these strains were oral isolates, with the remaining 10 isolated from IE patients ().As is typical, the IE isolates were from blood, except perhaps SK1.
In Streptococcus mutans, competence for genetic transformation and biofilm formation are dependent on the two-component signal transduction system ComDE together with the inducer peptide pheromone competence-stimulating peptide (CSP) (encoded by comC).
Here, it is shown that the same system is also required for expression of the nlmAB genes, which encode a two-peptide nonlantibiotic. Introduction. Streptococcus mutans is a principal microorganism contributing to the ubiquitous oral infectious disease dental caries (Loesche, ).Recent improvements in DNA sequencing platforms and intensified analysis of the oral microbiome have supported the ecological plaque hypothesis that describes the etiology of the development of the most common oral infectious.
The potential for a similar means of gene transfer in other streptococci is suggested by the finding of competence genes in many of the genome Canada) described the S. mutans system and presented the functional analysis of a new two-component involved in biofilm formation and acid resistance in Streptococcus mutans.
This chapter addresses how genomic sciences are revealing why Streptococcus mutans is such an effective caries pathogen in humans.
Recognizing the importance of central metabolism and acid production in pathogenesis by S. mutans, many laboratories began functional studies in the post-genomic era with a focus on gene regulation, stress tolerance, and biofilm formation.Candidate cgMLST target gene set.
We began by identifying genes that were well-suited to incorporation into our cgMLST scheme by filtering the S. mutans UA (GenBank accession number NC_) reference genome based upon minimum gene length, gene overlap, and start/stop codon led to the identification of 1, genes, and this number was further winnowed to 1, genes.Lemos, J.
A., Chen, Y. Y. & Burne, R. A. Genetic and physiologic analysis of the groE operon and role of the HrcA repressor in stress gene regulation and acid tolerance in Streptococcus mutans.